Objective: During the preparation of Zishen Yutai Pills, 5-Hydroxymethylfurfural may be used. To establish a HPLC method for determination of 5-HMF in Zishen Yutai pill and to provide the potential quality control standard of it. Methods: RP-HPLC method was applied with the chromatographic condition as follows: the chromatographic column was Phenomenex Gemini C18 column (250×4.6mm, 5μm), methanol-water (10:90) as the mobile phrase, the flow rate was 0.8mL/min, the detection wavelength was 284nm, the column temperature was 30°C, and the injection volume was 10μL. Results: The calibration curve was linear in the range of 2.16~43.27μg. mL-1 for 5-HMF (r2=0.9993). The RSD of the method repeatability was 0.97% (n=6) and the average recovery was 99.4% (RSD=0.58%). What’s more, the specificity, precision and stability results were also good. Conclusion: The method for HPLC is not only sensitive and accurate. It is also easy to operate which can be used as the determination method for 5-HMF and has a guiding role for the quality control of the Zishen Yutai pill.
| Published in | International Journal of Chinese Medicine (Volume 5, Issue 1) |
| DOI | 10.11648/j.ijcm.20210501.13 |
| Page(s) | 14-17 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2021. Published by Science Publishing Group |
HPLC, Zishen Yutai Pill, 5-HMF, Determination, Methodology
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APA Style
Lin Shaozhu, Huang Minna, Liu Hui, Huang Jiawen, Jin Weijun, et al. (2021). HPLC Method for the Determination Content of 5-HMF in Zishen Yutai Pill. International Journal of Chinese Medicine, 5(1), 14-17. https://doi.org/10.11648/j.ijcm.20210501.13
ACS Style
Lin Shaozhu; Huang Minna; Liu Hui; Huang Jiawen; Jin Weijun, et al. HPLC Method for the Determination Content of 5-HMF in Zishen Yutai Pill. Int. J. Chin. Med. 2021, 5(1), 14-17. doi: 10.11648/j.ijcm.20210501.13
AMA Style
Lin Shaozhu, Huang Minna, Liu Hui, Huang Jiawen, Jin Weijun, et al. HPLC Method for the Determination Content of 5-HMF in Zishen Yutai Pill. Int J Chin Med. 2021;5(1):14-17. doi: 10.11648/j.ijcm.20210501.13
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Download@article{10.11648/j.ijcm.20210501.13,
author = {Lin Shaozhu and Huang Minna and Liu Hui and Huang Jiawen and Jin Weijun and Wu Xiaosong},
title = {HPLC Method for the Determination Content of 5-HMF in Zishen Yutai Pill},
journal = {International Journal of Chinese Medicine},
volume = {5},
number = {1},
pages = {14-17},
doi = {10.11648/j.ijcm.20210501.13},
url = {https://doi.org/10.11648/j.ijcm.20210501.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcm.20210501.13},
abstract = {Objective: During the preparation of Zishen Yutai Pills, 5-Hydroxymethylfurfural may be used. To establish a HPLC method for determination of 5-HMF in Zishen Yutai pill and to provide the potential quality control standard of it. Methods: RP-HPLC method was applied with the chromatographic condition as follows: the chromatographic column was Phenomenex Gemini C18 column (250×4.6mm, 5μm), methanol-water (10:90) as the mobile phrase, the flow rate was 0.8mL/min, the detection wavelength was 284nm, the column temperature was 30°C, and the injection volume was 10μL. Results: The calibration curve was linear in the range of 2.16~43.27μg. mL-1 for 5-HMF (r2=0.9993). The RSD of the method repeatability was 0.97% (n=6) and the average recovery was 99.4% (RSD=0.58%). What’s more, the specificity, precision and stability results were also good. Conclusion: The method for HPLC is not only sensitive and accurate. It is also easy to operate which can be used as the determination method for 5-HMF and has a guiding role for the quality control of the Zishen Yutai pill.},
year = {2021}
}
TY - JOUR T1 - HPLC Method for the Determination Content of 5-HMF in Zishen Yutai Pill AU - Lin Shaozhu AU - Huang Minna AU - Liu Hui AU - Huang Jiawen AU - Jin Weijun AU - Wu Xiaosong Y1 - 2021/03/26 PY - 2021 N1 - https://doi.org/10.11648/j.ijcm.20210501.13 DO - 10.11648/j.ijcm.20210501.13 T2 - International Journal of Chinese Medicine JF - International Journal of Chinese Medicine JO - International Journal of Chinese Medicine SP - 14 EP - 17 PB - Science Publishing Group SN - 2578-9473 UR - https://doi.org/10.11648/j.ijcm.20210501.13 AB - Objective: During the preparation of Zishen Yutai Pills, 5-Hydroxymethylfurfural may be used. To establish a HPLC method for determination of 5-HMF in Zishen Yutai pill and to provide the potential quality control standard of it. Methods: RP-HPLC method was applied with the chromatographic condition as follows: the chromatographic column was Phenomenex Gemini C18 column (250×4.6mm, 5μm), methanol-water (10:90) as the mobile phrase, the flow rate was 0.8mL/min, the detection wavelength was 284nm, the column temperature was 30°C, and the injection volume was 10μL. Results: The calibration curve was linear in the range of 2.16~43.27μg. mL-1 for 5-HMF (r2=0.9993). The RSD of the method repeatability was 0.97% (n=6) and the average recovery was 99.4% (RSD=0.58%). What’s more, the specificity, precision and stability results were also good. Conclusion: The method for HPLC is not only sensitive and accurate. It is also easy to operate which can be used as the determination method for 5-HMF and has a guiding role for the quality control of the Zishen Yutai pill. VL - 5 IS - 1 ER -
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