Combination of HPLC Fingerprint and QAMS as a New Analytical Approach for Determination of Bufadienolides in Bufonis Venenum
Yi Luo,
Cejia Liu,
Shengci Fan,
Zhaoqing Meng,
Fei He
Issue:
Volume 4, Issue 1, March 2020
Pages:
1-11
Received:
28 November 2019
Accepted:
18 December 2019
Published:
21 January 2020
Abstract: The present study presents an effective and comprehensive evaluation method for assessing the quality of Bufonis Venenum, based on analysis of high performance liquid chromatography (HPLC) fingerprints in combination with similarity analysis, hierarchical cluster analysis (HCA), principal component analysis (PCA) and a quantitative analysis multi-components by single marker (QAMS) method. Nine common peaks identified by fingerprint detection results of 10 batches of samples were collected and used for the similarity analysis, HCA, PCA and QAMS analysis. These methods drew a similar conclusion that the quantitative analysis multi-components by single marker (QAMS) method 41 Bufonis Venenum samples were categorized into four main groups by HCA and PCA, and the majority of the samples with similar ingredients were mainly concentrated in Shandong area. When QAMS method was compared with the external standard method (ESM), it was feasible to evaluate the quality of Bufonis Venenum by the values of relative correction factors (RCFs) from cinobufagin (the internal reference) versus arenobufagin, bufalin, bufotalin, resibufogenin and telocinobufogin. In conclusion, these methods were successfully applied to identification of the origin and evaluation the quality of Bufonis Venenum. Therefore, these evaluation methods are promising to be widely applied in the quality control of Traditional Chinese Medicines (TCMs).
Abstract: The present study presents an effective and comprehensive evaluation method for assessing the quality of Bufonis Venenum, based on analysis of high performance liquid chromatography (HPLC) fingerprints in combination with similarity analysis, hierarchical cluster analysis (HCA), principal component analysis (PCA) and a quantitative analysis multi-c...
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Analysis of TCM Syndrome Types of Manic Disorder: Data from Clinical Therapy of TCM and Western Medicine
Sun Fengli,
Li Wei,
Yu Fang,
Song Xinyu,
Liu Jie,
Chen Fengpei,
Zhu Jianfeng,
Gao Zhihan,
Ma Yongchun,
Chen Jiong,
Xing Baoping,
Chen Song,
Jin Weidong,
Wang Zhiqiang
Issue:
Volume 4, Issue 1, March 2020
Pages:
12-15
Received:
22 December 2019
Accepted:
30 December 2019
Published:
14 February 2020
Abstract: Objective: to analyze the distribution characteristics and composition ratio of Chinese medicine syndrome in manic disease from the published literature on the combined treatment of manic mania by Chinese and Western medicine. Methods: to collect literature from Chinese database, to analyze and classify the cases that meet the standard combined treatment of Chinese and Western medicine, analyze the distribution of different syndromes and composition ratio. Results: 1385 cases were studied. Among them, 48.95% were phlegm fire disturbance to the mind, 20.50% were stagnation of phlegm and heat, 12.37% were liver and gall depression heat, 10.76% were qi stagnation and blood stasis, and 7.51% were fire injury Yin. Conclusion: The first three syndrome can cover more than 80% of the proportion. The contribution degree of the first three is also higher.
Abstract: Objective: to analyze the distribution characteristics and composition ratio of Chinese medicine syndrome in manic disease from the published literature on the combined treatment of manic mania by Chinese and Western medicine. Methods: to collect literature from Chinese database, to analyze and classify the cases that meet the standard combined tre...
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